{"id":13683,"date":"2026-03-11T09:39:21","date_gmt":"2026-03-11T09:39:21","guid":{"rendered":"https:\/\/www.mmj.mw\/?p=13683"},"modified":"2026-03-16T14:28:20","modified_gmt":"2026-03-16T14:28:20","slug":"role-and-mechanism-of-pcbp1-as1-in-hodgkin-lymphoma-for-patients-with-hodgkin-lymphoma","status":"publish","type":"post","link":"https:\/\/www.mmj.mw\/?p=13683","title":{"rendered":"Role and mechanism of PCBP1-AS1 in Hodgkin lymphoma for patients with Hodgkin lymphoma"},"content":{"rendered":"\n<p><strong>Bingyan Xu, Meihua Chen*<\/strong><\/p>\n\n\n\n<p>Departments of Hematology and Geriatrics, The Second People\u2019s Hospital of Changzhou, the Third Affiliated Hospital of Nanjing Medical University, Changzhou, 213000 Jiangsu, China.<\/p>\n\n\n\n<ul><li><strong>Corresponding Author<\/strong>: Meihua Chen;<strong> E-mail<\/strong>: meihua_chen2028@163.com<\/li><\/ul>\n\n\n\n<p><strong>Abstract<br>Background<\/strong><br>Hodgkin lymphoma (HL) is a malignant tumour of the lymphoid system with complex pathogenesis. Increasing evidence suggests that long non-coding RNAs (lncRNAs) participate in HL progression, yet the role of poly(C)-binding protein 1 antisense RNA 1 (PCBP1-AS1) remains unclear.<br><strong>Methods<\/strong><br>Human HL cell lines (KM-H2, L1236, L428 and L540) and germinal-centre B cells were analysed for PCBP1-AS1, miR-516b-5p and TRIM2 expression by RT-qPCR and western blotting. Loss- and gain-of-function assays, including CCK-8, colony-formation and Transwell assays, were used to assess cell proliferation, migration and invasion. RNA pull-down, luciferase reporter and RNA immunoprecipitation assays verified molecular interactions among PCBP1-AS1, miR-516b-5p and TRIM2.<br><strong>Results<\/strong><br>PCBP1-AS1 was markedly up-regulated in HL cells compared with germinal-centre B cells. Silencing PCBP1-AS1 significantly reduced HL-cell proliferation, colony formation, migration and invasion. Mechanistically, PCBP1-AS1 directly bound to miR-516b-5p, which was down-regulated in HL cells, thereby relieving the inhibitory effect of miR-516b-5p on its downstream target TRIM2. Inhibition of miR-516b-5p reversed the suppressive effects of PCBP1-AS1 knockdown, while TRIM2 overexpression abolished the inhibitory effects of miR-516b-5p up-regulation on HL-cell malignant phenotypes.<br><strong>Conclusion<\/strong><br>PCBP1-AS1 promotes HL progression by acting as a competing endogenous RNA that sponges miR-516b-5p to up-regulate TRIM2. The PCBP1-AS1\/miR-516b-5p\/TRIM2 axis may represent a promising molecular target for HL therapy.<\/p>\n\n\n\n<p><strong>Keywords:<\/strong> Hodgkin lymphoma, PCBP1-AS1, miR-516b-5p, TRIM2<\/p>\n\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link\" href=\"https:\/\/www.mmj.mw\/wp-content\/uploads\/2026\/03\/Hodgkin-lymphoma.pdf\">DOWNLOAD PDF<\/a><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Bingyan Xu, Meihua Chen* Departments of Hematology and Geriatrics, The Second People\u2019s Hospital of Changzhou, the Third Affiliated Hospital of Nanjing Medical University, Changzhou, 213000 Jiangsu, China. Corresponding Author: Meihua Chen; E-mail: meihua_chen2028@163.com AbstractBackgroundHodgkin lymphoma (HL) is a malignant tumour of the lymphoid system with complex pathogenesis. Increasing evidence suggests that long non-coding RNAs (lncRNAs) [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[139],"tags":[66],"_links":{"self":[{"href":"https:\/\/www.mmj.mw\/index.php?rest_route=\/wp\/v2\/posts\/13683"}],"collection":[{"href":"https:\/\/www.mmj.mw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.mmj.mw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.mmj.mw\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.mmj.mw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13683"}],"version-history":[{"count":3,"href":"https:\/\/www.mmj.mw\/index.php?rest_route=\/wp\/v2\/posts\/13683\/revisions"}],"predecessor-version":[{"id":13731,"href":"https:\/\/www.mmj.mw\/index.php?rest_route=\/wp\/v2\/posts\/13683\/revisions\/13731"}],"wp:attachment":[{"href":"https:\/\/www.mmj.mw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13683"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.mmj.mw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13683"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.mmj.mw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13683"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}