Development of rice farming: a cause of the emergence of multiple insecticide resistance in populations of Anopheles gambiae s.l and its impact on human health in Malanville, Bénin

YADOULETON Anges^1,2-3*, BADOU Yvette³, SANOUSSI Falilath³, HOUNKANRIN Gildas³,TCHIBOZO Carine³, ADEWUMI Praise³, BABA-MOUSSA Lamine⁴

1. Ecole Normale Supérieure de Natitingou ; Université Nationale des Sciences, Technologies, Ingénierie et Mathématiques (UNSTIM)
2. Centre de Recherche Entomologique de Cotonou
3. Laboratoire des Fièvres Hémorragiques Virales et des Arbovirus du Bénin
4. Laboratoire de Biologie et de Typage Moléculaire en Microbiologie/Département de Biochimie et de Biologie Cellulaire/Faculté des Sciences et Techniques/Université d’Abomey-Calavi/ 05 BP 1604 Cotonou, Benin

*Corresponding Author: YADOULETON Anges; E-mail:anges33@yahoo.fr

Abstract
Aim
The rise in rice production in the district of Malanville, Northen Benin, is a present concern, as it has resulted in the widespread usage of pesticides for crop protection. This could impact human health but also life cycle of Anopheles gambiae, the main vector of malaria.
Methods
Therefore, insecticide susceptibility bioassays were carried out on populations of An. gambiae s.l aged to 3-5 days old (two from areas where insecticide is highly used and other two areas of low insecticide use) and subjected to insecticide-impregnated papers (Permethrin 0.75%; deltamethrin 0.05%; DDT 4% and bendiocarb 0.1%) following WHO protocol. Polymerase Chain Reactions (PCRs) were used for the detection of Acethlylcholinestrase (Ace-1) and the knock down resistance (kdr) L1014F mutations in An. gambiae populations. Finally, indirect bioassays were conducted for the investigating on the factors affecting the life cycle of An. gambiae due to the use of pesticides.
Results
An. gambiae from the four sites were resistant to DDT (6 to 8% and 10 to 14% respectively from areas of high and low dose), pyrethroids (22 to 26% and 30 to 36% for permethrin, from areas of high and low dose respectively and 66 to 70% and 72 to 80% for deltamethrin, from high and low dose) but susceptible to carbamate. The kdr L1014F mutation was detected in An. gambiae populations (0.88 to 0.90 and 0.84 to 0.88 from high and low dose, respectively). The ace-1 was detected at low frequencies (<0.002). Bioassays on the impacts of the use of pesticides in the life cycle of An. gambiae showed that soil substrates with pesticides residues have a negative impact on the life cycle eggs of An. gambiae.
Conclusion
These findings confirmed the negative impacts of pesticides use in rice farming and its impacts on the life cycle of An. gambiae.

Key words: Insecticides, Rice, Resistance, life cycle, Anopheles gambiae, Benin